Experiment progress…   Leave a comment

We had to thaw a new batch of cells; the cultures we were using had lost their ENaC activity. Why this happens is unclear; qualitiatively, ‘bad cells’ grow faster than good ones and eventually overrun the culture. Our previous group at CWRU has spent (and is still spending) time manipulating the culture media to reproducibly abolish and regain ENaC function. So far, nothing we have done can restore ENaC from cells that have lost the activity. It is not clear what the origin of the problem is, and since trying to figure it out is a very time-consuming distraction, it’s a lot easier to simply thaw a new batch of cells. In any case, we have a new batch thawed and growing, and simply from the morphology we are hopeful that we will have a strong ENaC signal. At least we checked before using all those filters….

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Posted December 12, 2010 by resnicklab in Uncategorized

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