Experiment update… so far, so good.   Leave a comment

Today, the epithelial cells have been passaged onto their final resting place- after 4 expansions, we had enough cells to plate 120 small filters. The cells will grow to confluence, differentiate, and finally be killed; all the while subjecting them to fluid flow (or not) and measuring the transepithelial Sodium current. Once they are killed, the cells will be stained for a few different proteins of interest (polycystin-2, acetylated alpha-tubulin, STAT-6, etc), and the cells imaged and analyzed for cilium length and protein distribution.

In the end, we hope to answer some questions regarding the cellular response to fluid flow- for example, can cells become ‘acclimated’ to a certain velocity of flow?

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Posted December 27, 2010 by resnicklab in Physiology, Science

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